Verdinexor

SKU: orb1688808

Description

Verdinexor (KPT-335) is an orally bioavailable, selective inhibitor of the nuclear export protein XPO1/CRM1. It is utilized in preclinical research to study oncology, virology, and inflammation, demonstrating activity in both cellular assays and animal models of cancer and viral infection.

Research Area

Cell Biology, Infectious Disease & Virology, Pharmacology & Drug Discovery

Key Properties

• CAS Number: 1392136-43-4
• MW: 442.32
• Purity: 98.81%
• Formula: C₁₈H₁₂F₆N₆O
• SMILES: FC(F)(F)c1cc(cc(c1)C(F)(F)F)-c1ncn(\C=C/C(=O)NNc2ccccn2)n1
• Target: Apoptosis,RSV,CRM1
• Solubility: DMSO:82 mg/mL (185.39 mM);Ethanol:9 mg/mL (20.35 mM);10% DMSO+40% PEG300+5% Tween 80+45% Saline:3.3 mg/mL (7.46 mM)

Bioactivity

• In Vivo:
  In Jurkat,OCI-Ly3,OCI-Ly10,和CLBL1 cells,Verdinexor inhibited cell viability with IC50 of 0.3 nM, 2.1 nM, 41.8 nM, and 8.5 nM, respectively. In Primary Canine DLBCL Cells and CLBL1 expressed XPO1 and SINE, KPT-335 induces apoptosis. Verdinexorpotently inhibits a variety of influenza virus strains, including the H1N1 epidemic virus, the highly pathogenic H5N1 bird flu virus, and the recently emerging H7N9 strain.
• In Vitro:
  In a model of autosomal dominant polycystic kidney disease, intraperitoneal injection of Verdinexor (5 mg/kg) weakened cyst growth by inhibiting XPO1. Administering Verdinexor orally (25 mg/kg) twice daily reduced the incidence and mortality of lung disease associated with lethal influenza A virus type, primarily by decreasing the expression of pro-inflammatory cytokines in the lungs, which in turn reduced pulmonary viral titers, thereby exhibiting antiviral activity.
• Cell Research:
  Cell viability for lymphoid lines is determined by the MTS assay using CellTiter 96 AQueous One Solution Cell Proliferation Assay Kit. Briefly, for lymphoid cell lines, 5×104 cells (or 1×105 primary DLBCL cells) are cultured in 100 μL of complete medium in 96-well plates in the presence of SINE compounds. After 72 hours, 20 μL of MTS solution is added to each well and cells are incubated for another 4 hours before measuring absorbance at 490 nm using a Wallac Victor 1420 Multilabel Counter. The IC50 of SINE is calculated using Prism 6 software. For the non-lymphoid cell lines, 96 well plates are seeded in triplicate in 90 μL with 2500 cells/well of OSA16, 5000 cells/well of C2, and 2500 cells/well of 323610-3. Seeded plates are cultured overnight then treated the following day with 10 μL of KPT-214 in C10 media at concentrations of 0.0001, 0.01, 0.1, 1.0, and 10 μM. Plates are collected at 92 hours, centrifuged at 1300 rpm, and supernatant is removed by inverting plates on absorbent paper. Plates are then sealed and immediately placed at 80°C for a minimum of 12 hours. Plates are then thawed and CyQUANT Cell Proliferation Assay is performed following the manufacturer's protocol. Briefly, 200 μL of the diluted working CyQUANT solution is added to each well and protected from light. Fluorescence is the measured using a SpectraMax M2 microplate reader at 480 nm excitation and 520 nm emission. Results are represented as percent of control, or plotted to calculate IC50 values at 92 hours.(Only for Reference)

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CRM1, KPT335, KPT-335, KPT 335, Verdinexor

Compound Identifiers

PubChem CID

71492799