VDAC1 Antibody

SKU: orb150784

Description

Mouse Anti-Human VDAC1 Monoclonal IgG2a

Research Area

Cell Biology

Images & Validation

• Tested Applications: ICC, IF, IHC, WB
• Dilution Range: WB (1:1000)
• Reactivity: Human, Mouse, Rat
• Application Notes:
  1 µg/ml of SMC-456 was sufficient for detection of VDAC1 in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone No.: N152B/23 (Formerly sold as S152B-23)
• Immunogen: Fusion protein amino acids 1-283 (full-length) of human VDAC1. Mouse: 98% identity (279/283 amino acids identical). Rat: 98% identity (279/283 amino acids identical) > 60% identity with VDAC2 and VDAC3.
• Target: VDAC1
• Molecular Weight: 30kDa
• Purification: Protein G Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS pH 7.4, 50% glycerol, 0.1% sodium azide Storage buffer changes when conjugated
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Voltage Dependent Anion Channel 1, Porin, Voltage dependent anion selective channel protein 1, Voltage-dependent anion-selective channel protein 1, hVDAC1, MGC111064, Mitochondrial Porin, Outer mitochondrial membrane protein porin 1, Plasmalemmal porin, Porin 31HL, Porin 31HM, PORIN-31-HL, VDAC 1, VDAC, VDAC-1

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-VDAC1 Monoclonal Antibody, Clone N152B/23. Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-VDAC1 Monoclonal Antibody at 1:100 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) VDAC1 Antibody (D) Composite.

Western Blot analysis of Mouse Brain and Human RT-4 lysates showing detection of ~30 kDa VDAC1 protein using Mouse Anti-VDAC1 Monoclonal Antibody, Clone N152B/23. Lane 1: Molecular Weight Ladder. Lane A: Mouse Brain Lysate. Lane B: Human RT-4 Lysate. Block: 5% Skim milkin TBST. Primary Antibody: Mouse Anti-VDAC1 Monoclonal Antibody at 1:200 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: ~30 kDa.

Quick Database Links

Gene Symbol

VDAC1

UniProt

P21796

NCBI Gene ID

7416

RefSeq (Protein):NP_003365.1