VCAM-1, Human, mAb 1G11B1 Antibody

SKU: orb1108787

Description

Mouse monoclonal antibody to VCAM-1

Images & Validation

• Tested Applications: FC, Functional Studies, IF, IHC-Fr, IP
• Dilution Range: For immunohistochemistry, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.
• Reactivity: Human
• Application Notes:
  F: Cryostatic sections were fixed in acetone, and incubated with mAb at 10 µg/ml in PBS/0.1% tween20 FC: 1ug mAb per 1x105 cells FS: Stimulated EC were preincubated for 30' at 37°C with mAb and than used in adhesion assay with T-cells IP: precleared EC lysates were immunoprecipitated with mAB and analyzed by 7.5% SDS-PAGE under reducing conditions

Key Properties

• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Immunogen: Full length protein
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: 1 ml (100 µg/ml) 0.2 µm filtered antibody solution in PBS, containing 0.1% bovine serum albumin
• Buffer/Preservatives: 0.2 ml (100 µg/ml) 0.2 µm filtered antibody solution in PBS, containing 0.1% bovine serum albumin
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CD106, INCAM-100, L1CAM, MGC99561; DKFZp779G2333