VAV3 Antibody

• Catalog Number: orb1246802
• Category: Antibodies
• Description: VAV3 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: C-SGEQGTLKLPEK
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 100kDa band observed in Human Bone Marrow lysates (calculated MW of 97.8kDa according to NP_006104.4). Recommended concentration: 0.5-1.5ug/ml. Primary incubation 1 hour at room temperature.Immunohistochemistry:Paraffin embedded Human Spleen. Recommended concentration: 7.5ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: VAV3
• UniProt ID: Q9UKW4
• NCBI: NP_006104.4, NP_001073343.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: VAV3, vav 3 oncogene, vav 3 guanine nucleotide exc
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 100kDa band observed in Human Bone Marrow lysates (calculated MW of 97.8kDa according to NP_006104.4). Recommended concentration: 0.5-1.5ug/ml. Primary incubation 1 hour at room temperature.Immunohistochemistry:Paraffin embedded Human Spleen. Recommended concentration: 7.5ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1246802 (0.5 ug/ml) staining of Human Bobe Marrow lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1246802 (7.5 ug/ml) staining of paraffin embedded Human Spleen. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.

orb1246802 Negative Control showing staining of paraffin embedded Human Spleen, with no primary antibody.