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Catalog Number | orb344623 |
---|---|
Category | Antibodies |
Description | ULP1 antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, IF, WB |
Reactivity | Yeast |
Isotype | IgG |
Immunogen | This purified antibody was prepared from rabbit serum after repeated immunizations with recombinant yeast ULP-1 protein. |
Concentration | 5.0 mg/mL |
Dilution range | ELISA: 1:4,000 - 1:20,000, IF: User Optimized, WB: 1:500 - 1:2,000 |
Form/Appearance | Lyophilized |
Purity | This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum. Reactivity against ULP-1 from other sources or ULP-2 has not been determined. |
Conjugation | Unconjugated |
UniProt ID | Q02724 |
NCBI | 6325237 |
Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
Alternative names | rabbit anti-ULP1 antibody, rabbit anti-ULP-1 antib Read more... |
Note | For research use only |
Application notes | This purified polyclonal antibody reacts with yeast ULP-1 by western blot and ELISA. Although not tested, this antibody is likely functional in immunohistochemistry and immunoprecipitation. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 72.4 kDa in size corresponding to yeast ULP-1 by western blotting in the appropriate lysate or extract. |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of ULP-1 fusion protein (arrowhead) using ULP1 antibody
Western blot analysis of ULP-1 homologues from other sources (lanes 2 through 9) using ULP1 antibody
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