ULP1 Antibody

SKU: orb344623

Description

ULP1 antibody

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 1:4,000 - 1:20,000, WB: 1:500 - 1:2,000
• Reactivity: Yeast
• Application Notes:
  This purified polyclonal antibody reacts with yeast ULP-1 by western blot and ELISA. Although not tested, this antibody is likely functional in immunohistochemistry and immunoprecipitation. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 72.4 kDa in size corresponding to yeast ULP-1 by western blotting in the appropriate lysate or extract.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: This purified antibody was prepared from rabbit serum after repeated immunizations with recombinant yeast ULP-1 protein.
• Purity: This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum. Reactivity against ULP-1 from other sources or ULP-2 has not been determined.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 5.0 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

rabbit anti-ULP1 antibody, rabbit anti-ULP-1 antibody, Probable sentrin specific protease antibody, Ubiquitin Like Protease antibody

Western Blot of Rabbit Anti-ULP1 Antibody. (in duplicate) Lane 1: 50 ng ULP1CD. Lane 2: 25 ng ULP1CD. Lane 3: 12.5 ng ULP1CD. Lane 4: 6.25 ng ULP1CD. Lane 5: 3.13 ng ULP1CD. Blocking: 5% BLOTTO in PBST. Primary Antibody: Anti-ULP1 at 1:1000 dilution O/N at 4°C. Secondary Antibody: Goat Anti-Rabbit HRP 1:5000 RT for 30 min.

Western blot using Biorbyt's Affinity Purified anti-Yeast ULP-1 antibody shows detection of a truncated ULP-1 fusion protein (arrowhead). Increasing concentrations of yeast ULP-1 were run on a SDS-PAGE, transferred onto nitrocellulose, and blocked for 1 hour with 5% non-fat dry milk in TTBS, and probed overnight at 4°C with a 1:1000 dilution of anti-yULP-1 antibody in 5% non-fat dry milk in TTBS. Detection occurred using a 1:1000 dilution of HRP-labeled Donkey anti-Rabbit IgG for 1 hour at room temperature. A chemiluminescence system was used for signal detection (Roche) using a 3-sec exposure time.

Western blot using Biorbyt's Affinity Purified anti-Yeast ULP-1 antibody was used to confirm the specificity of the antibody. SDS-PAGE of 2 µg of ULP-1 homologues from other sources (lanes 2 through 9). After blocking for 1 hour with 5% non-fat dry milk in TTBS, the blot was probed overnight at 4°C with a 1:1000 dilution of anti-yULP1 antibody detected as above. This antibody is specific for yeast ULP1 and does not react with ULP1 from related sources including human SENP.

Quick Database Links

UniProt

Q02724

RefSeq:6325237