UGDH Recombinant Mouse Monoclonal Antibody

SKU: orb1172825

Description

UGDH Recombinant Mouse Monoclonal Antibody

Research Area

Amino Acid Metabolism, Cell Biology, Energy Metabolism, Oxidative Stress

Images & Validation

• Tested Applications: ICC, IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:1000-5000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:50-200, IF=1:100-500
• Reactivity: Human
• Predicted Reactivity: Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Recombinant
• Isotype: IgG1, Kappa
• Clone No.: B6A9
• Target: UGDH
• Molecular Weight: 55 kDa
• Purification: Affinity purified by Protein G
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

UGDH_HUMAN; UDP-glucose 6-dehydrogenase; EC:1.1.1.22; UDP-Glc dehydrogenase; UDP-GlcDH; UDPGDH; Uridine diphospho glucose dehydrogenase; UDP glucose 6 dehydrogenase; GDH; UGD; DEE84; EIEE84;

Blocking buffer: 5% NFDM/TBST, Primary Ab Dilution: 1:5000, Primary Ab incubation condition: 2 hours at room temperature, Secondary Ab: Goat Anti-Mouse IgG H&L (HRP), Lysate: 1: Hela, 2: HepG2, 3: Rat Liver, 4: F9, Protein loading quantity: 20 µg, Exposure time: 3 s, Predicted MW: 55 kDa, Observed MW: 55 kDa.

Cell line: HeLa, Fixative: 4% Paraformaldehyde, Permeabilization: 0.1% TritonX-100, Primary Ab Dilution: 1:50, Primary incubation condition: 4°C overnight, Secondary Ab: Goat Anti-Mouse IgG, Nuclear counter stain: DAPI (Blue), Comment: Color green is the positive signal for orb1172825.

Tissue: Human prostatic hyperplasia, Section type: Formalin fixed & Paraffin-embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary Ab Dilution: 1:100, Primary Ab incubation condition: 1 hour at room temperature, Secondary Ab: SP Kit (Mouse), Counter stain: Hematoxylin (Blue), Comment: Color brown is the positive signal for orb1172825.

Quick Database Links

Gene Symbol

UGDH