UFD1L Antibody

SKU: orb1257906

Description

UFD1L Antibody

Images & Validation

• Tested Applications: IF, IHC, WB
• Reactivity: Human, Mouse
• Application Notes:
  WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:100

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-307 of human UFD1L (NP_005650.2).
• Target: UFD1L
• Molecular Weight: Observed: 37kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid
• Concentration: batch dependent
• Disclaimer: For research use only

Alternative Names

UFD1L, ubiquitin recognition factor in ER-associated degradation protein 1, UB fusion protein 1, ubiquitin fusion degradation 1 like, ubiquitin fusion degradation protein 1 homolog

Western blot analysis of extracts of various cell lines, using UFD1L antibody (orb1257906) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Immunohistochemistry of paraffin-embedded human colon using UFD1L antibody (orb1257906) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of MCF7 cells using UFD1L antibody (orb1257906).

Quick Database Links

Gene Symbol

UFD1L

UniProt

Q92890