UBR2 Antibody

SKU: orb1249442

Description

UBR2 Antibody

Research Area

Cell Biology

Images & Validation

• Tested Applications: ELISA, IHC
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Mouse
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Preliminary experiments gave bands at approx 150kDa in Rat Testis lysates after 0.01µg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 199kDa according to Rat NP_001171542.1. The detected band was successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands).Immunohistochemistry:Paraffin embedded Brain (Cortex). Recommended concentration: 5µg/mL.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: SCPKSGGDKSRAPT
• Target: UBR2
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

UBR2, ubiquitin protein ligase E3 component n-recognin 2, C6orf133, DKFZp686C08114, KIAA0349, MGC71112, RP3-392M17.3, bA49A4.1, dJ242G1.1, dJ392M17.3, OTTHUMP00000016403, OTTHUMP00000016405, OTTHUMP00000039767, OTTHUMP00000039768, ubiquitin ligase E3 alpha-II

orb1249442 (5 ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Quick Database Links

Gene Symbol

UBR2

UniProt

Q8IWV8

RefSeq (Protein):NP_056070.1