UBE2W Antibody (C-term)

• Catalog Number: orb1936547
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB32985
• Tested applications: IHC-P, WB
• Predicted Reactivity: Zebrafish
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:1000, WB: 1:1000, IHC-P: 1:10~50
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 17331 Da
• Target: This UBE2W antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 105-133 amino acids from the C-terminal region of human UBE2W.
• UniProt ID: Q96B02
• NCBI: NP_060769.4, NP_001257944.1, NP_001001481.2
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Ubiquitin-conjugating enzyme E2 W, N-terminus-conj
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

All lanes: Anti-UBE2W Antibody (C-term) at 1:1000 dilution. Lane 1: SK-BR-3 whole cell lysate. Lane 2: T47D whole cell lysate. Lane 3: MOLT-4 whole cell lysate. Lane 4: MCF-7 whole cell lysate. Lane 5: PC-12 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 17 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-UBE2W Antibody (C-term) at 1:1000 dilution. Lane 1: SK-BR-3 whole cell lysate. Lane 2: T47D whole cell lysate. Lane 3: Molt-4 whole cell lysate. Lane 4: MCF-7 whole cell lysate. Lane 5: PC-12 whole cell lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 17 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

UBE2W Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of UBE2W Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.