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UBE2N Rabbit Polyclonal Antibody
SKU: orb574929
Description
Research Area
Epigenetics & Chromatin, Immunology & Inflammation, Molecular Biology, Protein Biochemistry
Images & Validation
−Item 1 of 4
| Tested Applications | IHC, WB |
|---|---|
| Reactivity | Human, Mouse |
| Predicted Reactivity | Bovine, Canine, Equine, Guinea pig, Rabbit, Rat, Sheep, Yeast, Zebrafish |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Immunogen | The immunogen is a synthetic peptide directed towards the middle region of human UBE2N |
| Target | UBE2N |
| Molecular Weight | 17kDa |
| Purification | Protein A purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| Concentration | 1.0 mg/ml |
| Disclaimer | For research use only |
Alternative Names
−UBC13, UbcH13, HEL-S-71, UbcH-ben, UBCHBEN; UBC13
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Sample Tissue: Mouse Spleen, Antibody dilution: 1 ug/ml.
Lanes: 1: 40 ng HIS-UBE2D1 protein, 2: 40 ng HIS-UBE2D2 protein, 3: 40 ng HIS-UBE2D3 protein, 4: 40 ng HIS-UBE2D4 protein, 5: 40 ng HIS-UBE2E1 protein, 6: 40 ng HIS-UBE2E2 protein, 7: 40 ng HIS-UBE2E3 protein, 8: 40 ng HIS-UBE2K protein, 9: 40 ng HIS-UBE2L3 protein, 10: 40 ng HIS-UBE2N protein, 11: 40 ng HIS-UBE2V1 protein, 12: 40 ng HIS-UBE2V2 protein. Primary Antibody dilution: 1:500, Secondary Antibody: Anti-rabbit-HRP, Secondary Antibody dilution: 1:50000, Gene Name: UBE2N.
Sample Type: Human brain stem cells, Primary Antibody dilution: 1:500, Secondary Antibody: Goat anti-rabbit Alexa-Fluor 594, Secondary Antibody dilution: 1:1000, Color/Signal Descriptions: UBE2N: Red DAPI:Blue, Gene Name: UBE2N.
WB Suggested Anti-UBE2N Antibody Titration: 1.25 ug/ml, Positive Control: HepG2 cell lysate, UBE2N is strongly supported by BioGPS gene expression data to be expressed in Human HepG2 cells.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
UBE2N Rabbit Polyclonal Antibody (orb574929)
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