UBE2J2 Antibody

SKU: orb345671

Description

Enzyme E2 J2 antibody

Images & Validation

• Tested Applications: ELISA, IP, WB
• Dilution range: ELISA: 1:5,000 - 1:25,000, WB: 1:500 - 1:3,000
• Reactivity: Human, Mouse, Rat
• Application Notes:
  This affinity purified antibody has been tested for use in ELISA, western blotting and immunoprecipitation. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 29 kDa in size corresponding to Ube2j2 protein by western blotting in the appropriate cell lysate or extract.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of the human Ube2j2 protein.
• Purity: This affinity purified antibody is directed against human Ube2j2 protein. The product was affinity purified from monospecific antiserum by immunoaffinity chromatography. A BLAST analysis was used to suggest cross-reactivity with Ube2j2 protein from human, horse, opossum, cattle, dog, mouse, rat, macaque, salmon, chicken, zebra finch, Xenopus and platypus based on 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Concentration: 0.91 mg/mL
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rabbit anti-UBE2J2 antibody, rabbit anti-E2 J2 antibody, rabbit anti-Ubiquitin conjugating enzyme E2J2 antibody, Non-canonical ubiquitin-conjugating enzyme 2, Ube2j2, E2 ubiquitin-conjugating enzyme J2, NCUBE-2

Most modifiers mature by proteolytic processing from inactive precursors ("a" = amino acid). Arrowheads point to the cleavage sites. Ubiquitin is expressed either as polyubiquitin or as a fusion with ribosomal proteins. Conjugation requires activating (E1) and conjugating (E2) enzymes that form thioesters (S) with the modifiers. Modification of cullins by RUB involves SCF(SKP1/cullin-1/F-box protein)/CBC(cullin-2/elonginB/elonginC)-like E3 enzymes that are also involved in ubiquitination. In contrast to ubiquitin, the UBLs do not seem to form multi-UBL chains. UCRP(ISG15) resembles two ubiquitin moieties linked head-to-tail. Whether HUB1 functions as a modifier is currently unclear. APG12 and URM1 are distinct from the other modifiers because they are unrelated in sequence to ubiquitin.

Western blot using Biorbyt's affinity purified anti-Ube2j2 antibody shows detection of Ube2j2 in 293 cells over-expressing Myc-Ube2j2 (Lane 1). Lane 2 contains lysate from mock-transfected 293 cells.

Quick Database Links

UniProt

Q8N2K1

RefSeq:251757431