UBC12 Recombinant Mouse Monoclonal Antibody

• Catalog Number: orb1173021
• Category: Antibodies
• Description: UBC12 Recombinant Mouse Monoclonal Antibody
• Species/Host: Mouse
• Clonality: Recombinant
• Clone Number: H6E1
• Tested applications: ICC, IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Human, Mouse, Rat
• Reactivity: Human, Mouse, Rat
• Isotype: IgG1/Kappa
• Antibody Type: Recombinant Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-1:1000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:50-1:200, IF=0
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: UBE2M
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blocking buffer: 5% NFDM/TBST, Primary Ab Dilution: 1:1000, Primary Ab incubation condition: 4°C overnight, Secondary Ab: Goat Anti-Mouse IgG H&L (HRP), Lysate: 1: HeLa, 2: HEK-293, 3: 4T1, 4: Raw264.7, Protein loading quantity: 20 µg, Exposure time: 30 s, Predicted MW: 21 kDa, Observed MW: 21 kDa.

Cell line: Ramos, Fixative: 4% Paraformaldehyde, Permeabilization: 0.1% TritonX-100, Primary Ab Dilution: 1:200, Primary incubation condition: 4°C overnight, Secondary Ab: Goat Anti-Mouse IgG, Nuclear counter stain: DAPI (Blue), Comment: Color green is the positive signal for orb1173021.

Tissue: Human liver cancer, Section type: Formalin fixed & Paraffin embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary Ab Dilution: 1:100, Primary Ab incubation condition: 1 hour at room temperature, Secondary Ab: SP Kit (Mouse), Counter stain: Hematoxylin (Blue), Comment: Color brown is the positive signal for orb1173021.

Tissue: Mouse kidney, Section type: Formalin fixed & Paraffin embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary Ab Dilution: 1:100, Primary Ab incubation condition: 1 hour at room temperature, Secondary Ab: SP Kit (Mouse), Counter stain: Hematoxylin (Blue), Comment: Color brown is the positive signal for orb1173021.

Tissue: Rat colon, Section type: Formalin fixed & Paraffin embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary Ab Dilution: 1:100, Primary Ab incubation condition: 1 hour at room temperature, Secondary Ab: SP Kit (Mouse), Counter stain: Hematoxylin (Blue), Comment: Color brown is the positive signal for orb1173021.