TXNDC5 Antibody

SKU: orb1249296

Description

TXNDC5 Antibody

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 50kDa plus faint 35kDa bands observed in Human Lymph Nodes lysates and 48kDa in Mouse Lymph Node and in Mouse Intestine lysates (calculated MW of 47.6kDa according to Human NP_110437.2, 46.4kDa to Mouse NP_663342.3) and of 36.2kDa according to Human NP_001139021.1. Recommended concentration: 0.1-0.3µg/mL. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Small Intestine. Recommended concentration: 3.75µg/mL.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-RDLESLREYVESQLQ
• Target: TXNDC5
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

TXNDC5, thioredoxin domain containing 5 (endoplasmic reticulum), ERP46, EndoPDI, Hcc-2, MGC3178, PDIA15, UNQ364, ER protein 46, endoplasmic reticulum protein ERp46, endoplasmic reticulum resident protein 46, endothelial protein disulphide isomerase, protein disulfide isomerase family A, member 15, thioredoxin domain-containing protein 5, thioredoxin related protein, thioredoxin-like protein p46

orb1249296 (0.1 ug/ml) staining of Human Lymph Nodes lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1249296 (0.1 ug/ml) staining of Mouse Lymph Nodes (A) and Mouse Intestine (B) lysates (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1249296 (3.75 ug/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer Ph 6, AP-staining.

Quick Database Links

Gene Symbol

TXNDC5

UniProt

Q8NBS9

RefSeq (Protein):NP_110437.2, NP_001139021.1