TXNDC5 Antibody

• Catalog Number: orb1249296
• Category: Antibodies
• Description: TXNDC5 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Predicted Reactivity: Bovine
• Reactivity: Human, Mouse
• Immunogen: The immunogen for this antibody is: C-RDLESLREYVESQLQ
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 50kDa plus faint 35kDa bands observed in Human Lymph Nodes lysates and 48kDa in Mouse Lymph Node and in Mouse Intestine lysates (calculated MW of 47.6kDa according to Human NP_110437.2, 46.4kDa to Mouse NP_663342.3) and of 36.2kDa according to Human NP_001139021.1. Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Small Intestine. Recommended concentration: 3.75ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: TXNDC5
• UniProt ID: Q8NBS9
• NCBI: NP_001139021.1, NP_110437.2
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: TXNDC5, thioredoxin domain containing 5 (endoplasm
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 50kDa plus faint 35kDa bands observed in Human Lymph Nodes lysates and 48kDa in Mouse Lymph Node and in Mouse Intestine lysates (calculated MW of 47.6kDa according to Human NP_110437.2, 46.4kDa to Mouse NP_663342.3) and of 36.2kDa according to Human NP_001139021.1. Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Small Intestine. Recommended concentration: 3.75ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1249296 (0.1 ug/ml) staining of Human Lymph Nodes lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1249296 (0.1 ug/ml) staining of Mouse Lymph Nodes (A) and Mouse Intestine (B) lysates (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1249296 (3.75 ug/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer Ph 6, AP-staining.