Tuberin (TSC2) Antibody (S1798)

SKU: orb1930073

Description

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Signal Transduction

Images & Validation

• Tested Applications: IF, IHC-P, WB
• Dilution Range: IF - 1:10-50, WB - 1:1000, IHC-P - 1:10-50
• Reactivity: Human

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: BTD35515
• Immunogen: This Tuberin (TSC2) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1776-1805 amino acids from human Tuberin (TSC2). Antigen Region: 1776-1805 aa.
• Target: TSC2 {ECO:0000303|PubMed:7558029, ECO:0000312|HGNC:HGNC:12363}
• Molecular Weight: 200608 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Tuberin, Tuberous sclerosis 2 protein, TSC2, TSC4

Fluorescent confocal image of Hela cell stained with Tuberin (TSC2) Antibody (S1798).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with Tuberin (TSC2) primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min).Tuberin (TSC2) immunoreactivity is localized to Cytoplasm significantly.

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with TSC2 Antibody (S1798), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Western blot analysis of TSC2-pS1798 in Ramos cell line lysates (35 ug/lane). TSC2 (arrow) was detected using the purified Pab.

Western blot analysis of lysates from PC-3, LNCaP, Hela cell line (from left to right), using Tuberin (TSC2) Antibody. Diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35 ug per lane.

Western blot analysis of lysates from SH-SY5Y, MCF-7, PC-3, LNCaP, Hela, cell line (from left to right), using Tuberin (TSC2) Antibody. Diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35 ug per lane.

Quick Database Links

Gene Symbol

TSC2 {ECO:0000303|PubMed:7558029, ECO:0000312|HGNC:HGNC:12363}

UniProt

P49815

RefSeq (Protein):NP_001107854.1, NP_001070651.1, NP_000539.2