TSLP Antibody Biotin Conjugated

SKU: orb345188

Description

TSLP antibody (Biotin)

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 1:20,000 - 1:50,000, WB: 1:1000-1:2000
• Reactivity: Human
• Application Notes:
  TSLP purified antibody has been tested in western blotting and suitable in ELISA. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 18 kDa in size corresponding to the human TSLP protein by western blotting in appropriate cell lysate or extract.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Anti-TSLP antibody was prepared from rabbit antiserum after repeated immunizations with a truncated form of recombinant human TSLP protein from E. coli.
• Purity: Anti-TSLP is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. This antibody is specific for human TSLP protein. A BLAST analysis was used to suggest cross-reactivity with TSLP from human sources based on 100% homology with the immunizing sequence. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin and anti-Rabbit Serum. Cross-reactivity with TSLP from other sources has not been determined.
• Conjugation: Biotin

Storage & Handling

• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/mL
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rabbit anti-TSLP biotin conjugated antibody, rabbit anti-Thymic Stromal Lymphopoietin biotin conjugated antibody

Western blot of Biotin conjugated Rabbit Anti-TSLP primary antibody. Lane 1: Human TSLP. Lane 2: None. Load: 50 ng per lane. Primary antibody: TSLP Biotin conjugated antibody at 1:1000 for 60 min at RT. Secondary antibody: Peroxidase streptavidin secondary antibody at 1:40000 for 30 min at RT. Blocking: orb348637 for 30 min at RT. Observed/Predicted size: 13 kDa, 13 kDa for Human TSLP. Other band(s): None.

Quick Database Links

UniProt

Q969D9

RefSeq (Protein):NP_149024.1