PRSS2 Antibody (HRP)

SKU: orb344910

Description

Trypsinogen antibody (Peroxidase)

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 1:10,000 - 1:40,000, WB: 1:1,000 - 1:5,000
• Reactivity: Bovine
• Application Notes:
  Anti-TRYPSINOGEN Peroxidase Conjugated Antibody has been tested by ELISA and Western blot. This product is assayed against 1.0 ug of Trypsinogen [Bovine Pancreas] in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:3,000 of the reconstitution concentration is suggested for this product. Specific conditions should be optimized by user.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Trypsinogen [Bovine Pancreas]
• Target: PRSS2
• Purity: Anti-TRYPSINOGEN (RABBIT) Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Trypsinogen [Bovine Pancreas]. Cross reactivity against Trypsinogen from other tissues and species may occur but have not been specifically determined.
• Conjugation: HRP

Storage & Handling

• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/mL
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rabbit anti-Trypsinogen Antibody HRP Conjugation, Peroxidase Conjugated rabbit anti-Trypsinogen Antibody, Brain trypsinogen antibody, Cationic trypsinogen antibody, Mesotrypsinogen antibody, Pancreatic anionic trypsinogen antibody, Protease, serine, 2 antibody, PRSS2 antibody

Western Blot of Peroxidase conjugated Rabbit Anti-Trypsinogen primary antibody. Lane 1: Trypsinogen. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Peroxidase rabbit secondary antibody at 1:1000 for 60 min at RT. Block: orb348637 for 30 min at RT. Predicted/Observed size: 24 kDa, 24 kDa for Trypsinogen. Other band(s): None.

Quick Database Links

Gene Symbol

PRSS2

UniProt

Q29463

RefSeq:AAI42037.1