THPO Antibody

SKU: orb1671463

Description

THPO Antibody

Research Area

Cell Biology

Images & Validation

• Tested Applications: ELISA, WB
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hTPO. Anti-Human TPO specific antibody was purified by affinity chromatography employing immobilized hTPO matrix.
• Target: THPO
• Purification: TPO specific antibody was purified by affinity chromatography employing immobilized hTPO matrix
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Thrombopoietin, Megakaryocyte colony-stimulating factor, c-MPL Ligand, MGDF

To detect hTPO by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human TPO as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hTPO.

To detect hTPO by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hTPO is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Quick Database Links

Gene Symbol

THPO

UniProt

P40225

RefSeq:P40225