Tph2 Antibody

SKU: orb1249325

Description

Tph2 Antibody

Research Area

Metabolism Research

Images & Validation

• Tested Applications: ELISA, IHC
• Reactivity: Mouse
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Preliminary experiments gave an approx 85kDa band in Mouse fetal Brain lysates after 0.3µg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 55.9kDa according to NP_775567.2. The 85kDa band was successfully blocked by incubation with the immunizing peptide.Immunohistochemistry:In paraffin embedded Mouse Brain stem shows staining of Raphe nucleii serotonergic neurons consistent with observation by different antibodies. Recommended concentration, 0.5-2µg/mL.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-SLTQNKAIKSEDK
• Target: Tph2
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Tph2, tryptophan hydroxylase 2, AU043594, MGC159133, Ntph, OTTMUSP00000022885, neuronal tryptophan hydroxylase, tryptophan 5-hydroxylase 2, tryptophan 5-monooxygenase 2

orb1249325 (0.5 ug/ml) staining of paraffin embedded Mouse Brain stem in sagittal (left) and coronal (right) sections of Raphe nuclei. Detection Alexa 594.

Quick Database Links

Gene Symbol

Tph2

UniProt

Q8CGV2

RefSeq (Protein):NP_775567.2