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Description
Research Area
Images & Validation
−| Tested Applications | FC, ICC, IF, IHC-Fr, IHC-P, IP, WB |
|---|---|
| Dilution Range | WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 IP: 1/20 FC: 1/50-1/100 |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | A synthetic peptide of human TNF Receptor II |
| Target | TNFRSF1B |
| Molecular Weight | Calculated MW: 48 kDa; Observed MW: 65 kDa |
| Purification | Affinity Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% rAlbumin. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
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Western blot analysis of TNF Receptor II in 3T3, C6 lysates using TNF Receptor 2 antibody.

Western blot analysis of TNF Receptor II in Jurkat, Hela lysates using TNF Receptor II antibody.

Immunocytochemistry analysis of TNF Receptor II (green) in Hela using TNF Receptor II antibody, and DAPI (blue).

Immunohistochemistry analysis of paraffin-embedded Human lung cancer using TNF Receptor II antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
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Protocol Information
TNF Receptor 2 Rabbit pAb (orb1565581)
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