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Description
Research Area
Images & Validation
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| Tested Applications | FC, Functional Studies, IA, WB |
|---|---|
| Dilution Range | For flow cytometry and Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10. For neutralization of biological activity dilutions have to be made according to the amounts TNF-alpha to be inactivated. Before use in biological assays, the product must be filter sterilized and depending on the concentration to be used dialyzed against culture medium to remove the sodium azide added. Please inquire for availability of azide free solutions. |
| Reactivity | Human |
Key Properties
−| Clonality | Polyclonal |
|---|---|
| Isotype | Rabbit Ig |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | 1 ml (100 µg/ml) 0.2 µm filtered protein A purified antibody solution in PBS, containing 0.1% bovine serum albumin. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
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Protocol Information
TNF-alpha, Human, pAb Antibody (orb1108737)
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