TLR9 Antibody

SKU: orb248106

Description

The mammalian immune system is composed of two components: innate immunity and adaptive immunity. Toll-like receptors (TLRs) are an essential part of the innate immune system. Through recognition of pathogen-associated molecular patterns (PAMPs), TLRs mount an immune defense against pathogens. There are 10 mammalian TLRs (TLR1-10). Unmethylated CpG sequences in bacterial genomes and viral DNA molecules activates TLR9, leading to the inflammatory response.

Research Area

Cell Biology

Images & Validation

• Tested Applications: FACS, IHC-P, WB
• Dilution Range: Western blot: 2-4ug/ml,Flow cytometry: 1ug/10^6 cells,Immunohistochemistry (FFPE): 5ug/ml
• Reactivity: Human, Mouse
• Application Notes:
  Provided assay concentrations are suggestions only, antibody titration may be required for optimal results.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1, kappa
• Clone No.: TR9541
• Immunogen: A recombinant protein fragment from the N-terminal portion of human TLR9 was used as the immunogen for this antibody.
• Purification: Protein G Affinity Chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.5 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin, 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Western blot testing of human samples with TLR9 antibody at 2 ug/ml.

Western blot testing of mouse samples with TLR9 antibody at 2 ug/ml.

IHC testing of FFPE human stomach tissue with TLR9 antibody at 5 ug/ml. HIER: steam tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.

FACS testing of human PBMC (lymphocytes) with TLR9 antibody at 0.5ug/10^6 cells. Green: isotype control; Red: TLR9 antibody.