TIMP4 Antibody

SKU: orb344479

Description

TIMP4 antibody

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: ELISA: 1:20,000 to 1:100,000, IHC: 5-10µg/mL, WB: 1:2,000 to 1:10,000
• Reactivity: Human
• Application Notes:
  Anti-TIMP4 antibody has been tested for use in ELISA, Native - Western, and IHC (P). SDS-PAGE - Western Blot not useful for endogenous detection. Specific conditions for reactivity should be optimized by the end user.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone No.: 9:4-7
• Immunogen: Anti-TIMP4 (MOUSE) Monoclonal Antibody was produced in mouse by repeated immunizations with full length human TIMP4 protein followed by hybridoma development.
• Target: TIMP4
• Purity: Anti-TIMP4 was purified from clarified mouse ascetic fluid by Protein A chromatography followed by extensive dialysis against the buffer stated above. TIMP4 antibody is specific for human TIMP4 protein. This antibody reacts with human TIMP4 in tissue sections of formalin-fixed, paraffin-embedded In ELISAs and Native-PAGE, this antibody does not cross-react with recombinant human TIMP-1, -2 or -3. A BLAST analysis was used to suggest cross-reactivity with TIMP4 from human and mouse sources based on the immunizing sequence. Cross-reactivity with TIMP-4 from other sources has not been determined.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/mL
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

mouse anti-TIMP4 antibody, Tissue inhibitor of metalloproteinase-4, Metalloproteinase inhibitor 4, TIMP-4, TIMP 4, TIMP4 antibody, anti-TIMP-4 antibody

Immunohistochemistry of Mouse Anti-TIMP4 Antibody. Tissue: human breast carcinoma. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: a) isotype specific IgG2bk monoclonal; b) monoclonal TIMP-4 antibody (clone 9:4-7); and c) polyclonal TIMP-4, each at 10 µg/ml. Secondary antibody: Peroxidase secondary antibody at 1:10000 for 45 min at RT. Localization: TIMP-4 is secreted. Staining: TIMP-4 as precipitated brown signal.

Immunohistochemistry of Mouse anti-TIMP4 antibody. Tissue: human small intestine. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: anti-TIMP4 antibody at 10 µg/ml for 1 h at RT. Secondary antibody: Peroxidase mouse secondary antibody at 1:10000 for 45 min at RT. Staining: TIMP4 as precipitated red signal with hematoxylin purple nuclear counterstain.

Immunohistochemistry of Mouse anti-TMIP4 antibody. Tissue: human kidney. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: anti-TIMP4 antibody at 10 µg/ml for 1 h at RT. Secondary antibody: Peroxidase mouse secondary antibody at 1:10000 for 45 min at RT. Staining: TIMP4 as precipitated red signal with hematoxylin purple nuclear counterstain.

Quick Database Links

Gene Symbol

TIMP4

UniProt

Q99727

RefSeq (mRNA):NM_003256.3