TIMP2 Antibody

SKU: orb1537245

Description

TIMP2 Antibody

Images & Validation

• Tested Applications: ELISA, IHC-P, WB
• Dilution Range: ELISA, IHC-P (10 - 15 µg/ml), WB (1 - 2 µg/ml)
• Reactivity: Bovine, Canine, Equine, Gallus, Guinea pig, Hamster, Human, Monkey, Mouse, Porcine, Rabbit, Rat, Sheep, Zebrafish
• Application Notes:
  Further information: Immunohistochemistry: This was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration was determined to be 10 ug/ml.

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone No.: F27P3A4
• Immunogen: Synthetic peptide from human TIMP2. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Marmoset, Mouse, Rat, Sheep, Bovine, Dog, Hamster, Elephant, Panda, Horse, Rabbit, Pig, Opossum, Guinea pig, Turkey, Chicken, Lizard, Seabass, Zebrafish (100%); Xenopus, Medaka, Pufferfish (91%); Bat, Stickleback (82%).
• Target: TIMP2
• Purification: Affinity purified from tissue culture supernant
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 10 mM PBS, pH 7.2, 0.09% sodium azide.
• Concentration: 0.5 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

TIMP2, Metalloproteinase inhibitor 2, TIMP-2, CSC-21K

Western blot of recombinant human TIMP-2 protein using anti-TIMP-2, clone F27P3A4.

Quick Database Links

Gene Symbol

TIMP2