Thymidine

SKU: orb1296048

Description

Thymidine

Research Area

Cell Biology, Infectious Disease & Virology, Metabolism Research, Molecular Biology

Key Properties

• CAS Number: 50-89-5
• MW: 242.23
• Purity: 99.95%
• Formula: C₁₀H₁₄N₂O₅
• SMILES: O=C1N([C@@H]2O[C@H](CO)[C@@H](O)C2)C=C(C)C(=O)N1
• Target: DNA/RNA Synthesis,Virus Protease,Endogenous Metabolite
• Solubility: Pyridine, Methanol, etc.:Soluble;DMSO:245 mg/mL (1011.44 mM);5% DMSO+95% Saline:2.63 mg/mL (10.86 mM);10% DMSO+90% Saline:5 mg/mL (20.64 mM);Saline:25 mg/mL (103.21 mM);Ethanol:< 1 mg/mL (insoluble)

Bioactivity

• In Vivo:
  METHODS: To investigat the effects of folate analogs on superantigen Reactive peripheral T cells in vivo Thymidine (500 mg/kg) was injected intraperitoneally into MTX- or tomudex-administered mice twice daily for ten days. Results: Both MTX and tomudex blocke the expansion and accelerate the peripheral clearance of Vβ8 Selective T cells. Thymidine administration completely eliminated this effect, suggesting that Inhibition of glycoside synthase (TS), but not Other folate-dependent enzymes, in the predominant mechanism.
• In Vitro:
  METHODS: Human hepatocellular carcinoma cells HepG2 were treated with Thymidine (300-1200 µM) and H2O2 (1 mM for 0.5 h. DNA damage was detected by comet assay. Results: Fo the control and Thymidine alone groups the Samples appeared almost circular due to the absence of DNA damage. After exposure to 1.0 mM H2O2 for 0.5 h, a clear comet was observed, indicating DNA damage in this group. however, there was a decreasing trend in the extent of DNA damage in the three combination groups a the concentration of Thymidine pretreatment increased. METHODS: Jurkat, MOLT4 and NALM6 cells were treated with Thymidine (2 mM for 18 h. RESULTS: Thymidine arrest the cell cycle a the G1/S boundary.

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Disclaimer: For research use only

Alternative Names

Endogenous Metabolite, EndogenousMetabolite, Inhibitor, inhibit, NSC 21548, NSC21548, NSC-21548, arrest, 5-Methyldeoxyuridine, Deoxyribothymidine, DNA, DThyd, DNA Synthesis, DNA/RNA Synthesis, DNASynthesis, synchronizing, replication, RNA Synthesis, RNASynthesis, Thymidin, Thymidine, precursor

Compound Identifiers

PubChem CID

5789