Tetrahydrofolic acid

SKU: orb2901503

Description

Tetrahydrofolic acid (L-5,6,7,8-Tetrahydrofolic acid) is the biologically active vitamin B9 folate derivative. Tetrahydrofolic acid is a donor of one-carbon groups for amino acids, nucleic acids, and lipids. Tetrahydrofolic acid serves as an acceptor of free formaldehyde, producing 5,10-methylenetetrahydrofolate-Tetrahydrofolic acid.

Key Properties

• CAS Number: 135-16-0
• MW: 445.43
• Purity: >98% (HPLC)
• Formula: C₁₉H₂₃N₇O₆
• SMILES: Nc1nc2NCC(CNc3ccc(cc3)C(=O)N[C@@H](CCC(O)=O)C(O)=O)Nc2c(=O)[nH]1
• Target: Endogenous Metabolite
• Solubility: In Vitro: DMSO : 20.83 mg/mL (46.76 mM; Ultrasonic )

Bioactivity

• In Vivo:
  Animal model: Adh5-/-mice. Dosage: 62.5 mg/kg. Administration: Intraperitoneal injection; daily. Result: Perturbed hematopoiesis, increased ser139-H2AX phosphorylation, and decreased the survival of progenitor cells (HSPCs).
• In Vitro:
  Cell Viability Assay Cell line: Adh5-/- DT40 cells. Concentration: 0-200 μM. Incubation time: 3 days. Result: Viability of Adh5-/- DT40 cells rapidly dropped. Western blot analysis. Cell line: Adh5-/- DT40 cells. Concentration: 0-200 μM. Incubation time: 16 hours. Result: Strongly promoted FANCD2 and ser139-H2AX focus formation in Adh5-/- cells in a dose-dependent manner.

Storage & Handling

• Storage: Storage temperature: -20°C. Stability: ≥ 2 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Quick Database Links

Gene Symbol

Endogenous Metabolite