Tetrahydrofolic acid

SKU: orb1705104

Description

Tetrahydrofolic acid is the biologically active form of folate (vitamin B9), serving as an essential one-carbon unit donor in cellular metabolism. It is widely used in biochemical research to study nucleotide synthesis, amino acid metabolism, and related pathways in both cell-based and enzymatic assays.

Research Area

Metabolism Research

Key Properties

• CAS Number: 135-16-0
• MW: 445.43
• Purity: 99.78% (May vary between batches)
• Formula: C₁₉H₂₃N₇O₆
• SMILES: Nc1nc2NCC(CNc3ccc(cc3)C(=O)N[C@@H](CCC(O)=O)C(O)=O)Nc2c(=O)[nH]1
• Target: Endogenous Metabolite
• Solubility: DMSO:4.46 mg/mL (10.01 mM);H2O:1 mg/mL (2.25 mM);10% DMSO+40% PEG300+5% Tween 80+45% Saline:1 mg/mL (2.25 mM)

Bioactivity

• In Vivo:
  In Adh5-/- mice, Tetrahydrofolic acid (62.5 mg/kg; intraperitoneal injection; daily) resulted in disrupted hematopoiesis, increased phosphorylation of Ser139-H2AX, and decreased survival of hematopoietic stem and progenitor cells (HSPCs).
• In Vitro:
  In Adh5-/- DT40 cells, Tetrahydrofolic acid (0-200 µM; 3 days) resulted in a rapid drop in cell viability.

Storage & Handling

• Storage: store under nitrogen,keep away from direct sunlight,store at low temperature | Powder: -20°C for 3 years | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Endogenous Metabolite, EndogenousMetabolite, folate-H4, 5,6,7,8-tetrahydrofolic acid, Tetrahydropteroylglutamic acid, Tetrahydrofolic acid, th-folate

Compound Identifiers

PubChem CID

135444742