Tenascin C Antibody

• Catalog Number: orb749579
• Category: Antibodies
• Description: In Western blotting, it reacts with two bands of ~MW of 210kDa and 300kDa, identified as two isoforms of Tenascin C. Specificity of this mAb is validated by sequential immunoprecipitation with a PAb against Tenascin C. Tenascin C is a multifunctional, disulfide-linked hexameric extracellular matrix glycoprotein expressed in association with mesenchymal epithelial interactions during development and in the neo-vasculature and stroma of undifferentiated tumors. In adults, it is restricted to certain epithelial-stromal interfaces and increases markedly in hyper-proliferative diseases and in stroma of many neoplasms, including gliomas, breast, squamous and lung carcinomas.
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: Mouse IgG1, kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Immunogen: Human breast carcinoma cells were used as the immunogen for the Tenascin C antibody.
• UniProt ID: P24821
• Tested applications: IHC-P
• Dilution range: Immunohistochemistry (FFPE): 2-4ug/ml for 30 min at RT
• Application notes: Optimal dilution of the Tenascin C antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Antibody Type: Primary Antibody
• Clone Number: T2H5
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Hazard Information: This Tenascin C antibody is available for research use only.
• Note: For research use only

IHC: Formalin-fixed, paraffin-embedded human lung carcinoma stained with Tenascin C antibody (T2H5) at 4 ug/ml. Antigen retrieval in 10mM Tris with 1mM EDTA, pH9.0; ABC detection system with DAB Chromogen. Note staining of connective tissue.