TAF10 Antibody

SKU: orb420371

Description

TAF10 antibody

Images & Validation

• Tested Applications: DOT, ELISA, WB
• Dilution Range: WB: 1.0 ug/ml
• Reactivity: Human
• Application Notes:
  Anti-TAF10 Antibody has been tested for use in ELISA, Dot Blot, and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 21.7 kDa in size corresponding to TAF10 protein by western blotting in the appropriate cell lysate or extract.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: TAF10 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to C-Terminal portion of human TAF10.
• Purity: This affinity-purified antibody is directed against human TAF10 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest cross reactivity with TAF10 protein from human and mouse (100% homology). Reactivity against homologues from other sources is not known.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.08 mg/ml
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rabbit anti-TAF10 antibody, rabbit anti-Transcription initiation factor TFIID subunit 10 antibody, TAF-10, TAF 10, TAF2A, TAF2H, TAFII30, STAF28, Transcription initiation factor TFIID 30 kDa subunit, TAF(II)30, TAFII-30

Western Blot of Rabbit Anti-TAF10 antibody. Lane 1: Opal Pre-stained Ladder. Lane 2: Hela WCL (p/n orb348668). Lane 3: HeLa nuclear extract (p/n orb348671). Lane 4: HEK293 WCL (p/n orb348669). Lane 5: PANC-1 WCL (p/n orb348710). Lane 6: Mouse brain WCL. Lane 7: Opal Pre-stained Ladder. Load: 35 µg per lane. Primary antibody: TAF10 antibody at 1:1000 for overnight at 4°C. Secondary antibody: rabbit secondary HRP antibody (p/n orb347654) at 1:70000 for 45 min at RT. Block: (p/n orb348637) overnight at 4°C. Predicted/Observed size: 21 kda for TAF10.

Quick Database Links

UniProt

Q12962

RefSeq (Protein):NP_006275.1