TACSTD2 / Trop-2 antibody

SKU: orb2276355

Description

TACSTD2 / Trop-2 antibody validated for immunohistochemistry on 76 different Normal Tissues

Images & Validation

• Tested Applications: IHC
• Dilution Range: 1:100-1:200
• Reactivity: Human
• Application Notes:
  Positive Control: Liver: A strong membranous Trop-2 immunostaining should be seen in bile ducts. Negative Control: Liver: Hepatocytes should not show any Trop-2 immunostaining. Cellular Localization: Cell Surface Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.

Key Properties

• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone No.: MSVA-733R
• Immunogen: Recombinant fragment of human TACSTD2 protein (around aa 31-274) (exact sequence is proprietary)
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Cell surface glycoprotein Trop-2; Membrane Component Chromosome 1, Surface Marker 1 (M1S1); Pancreatic Carcinoma Marker Protein GA733-1; TROP2; Tumor-Associated Calcium Signal Transducer 2 (TACSTD2)

Kidney cortex In the kidney Trop 2 immunostaining is strong in distal tubuli and collecting ducts but weaker in the parietal layer of the Bowman capsule.

Liver Strong Trop 2 staining in intrahepatic bile ducts of the liver.

Strong Trop 2 immunostaining of a triple negative breast cancer of no special type NST.

Quick Database Links

UniProt

P09758