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Swine IgG F(ab')2

SKU: orb2652737

Description

Swine IgG F(ab')2

Research Area

Protein Biochemistry

Images & Validation

Tested ApplicationsSDS-PAGE
Application Notes
Swine IgG F(ab')2 Fragment has been tested by SDS-Page and are ideal for ELISA, western blotting, Immunohistochemistry, as well as other antibody detection methods

Key Properties

SourceSwine
Biological OriginSwine
IsotypeIgG F(ab')2
ConjugationUnconjugated
PurityThis product was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by pepsin digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Swine IgG, anti-Swine IgG F(ab’)2 and anti-Swine Serum. No reaction was observed against anti-Swine IgG F(c) or anti-Pepsin.

Storage & Handling

StorageStore vial at 4° C prior to opening. This product is stable 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
Form/AppearanceLiquid (sterile filtered)
Buffer/PreservativesPreservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Concentration2.0 mg/mL
Expiration Date6 months from date of receipt.
DisclaimerFor research use only

Alternative Names

Swine IgG F(ab')2 fragment, Swine IgG Fab2 fragment

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Protocol Information

Protein Handling and Storage Guide
Protein Handling Guide
View Guide
SDS-PAGE
Sodium Dodecyl Sulphate PolyAcrylamide Gel Electrophoresis
View Protocol

Swine IgG F(ab')2 (orb2652737)

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