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Catalog Number | orb347975 |
---|---|
Category | Antibodies |
Description | Swine IgG F(ab')2 antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, IHC, WB |
Reactivity | Porcine |
Isotype | IgG |
Immunogen | Swine IgG F(ab')2 fragment |
Concentration | 4.6 mg/mL |
Dilution range | ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000 |
Form/Appearance | Liquid (sterile filtered) |
Purity | This product was prepared from monospecific antiserum by immunoaffinity chromatography using Swine IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Swine IgG, Swine IgG F(ab’)2 and Swine Serum. No reaction was observed against Swine IgG F(c). |
Conjugation | Unconjugated |
Storage | Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. |
Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
Alternative names | Rabbit Anti Swine IgG F(ab')2 Antibody, Rabbit Ant Read more... |
Note | For research use only |
Application notes | Anti-Swine IgG F(ab’)2 antibody has been tested by ELISA and is suitable for use in ELISA, immunohistochemistry, and western blot. Specific conditions for reactivity should be optimized by the end user. |
Expiration Date | 12 months from date of receipt. |
TRITC | |
This product was prepared from normal serum by delipidation, salt fractionation, ion exchange chromatography followed by pepsin digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Swine IgG, anti-Swine IgG F(ab’)2 and anti-Swine Serum. No reaction was observed against anti-Swine IgG F(c) or anti-Pepsin. | |
Swine |
IP | |
Agarose | |
This product is an purified Swine IgG isolated from normal Swine Serum digested by the enzyme pepsin and purified by chromatography coupled to activated agarose. Sufficient antibody capacity is provided to bind a minimum of 5.0 of anti-Swine IgG. | |
Swine |
SDS-PAGE | |
Unconjugated | |
This product was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by pepsin digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Swine IgG, anti-Swine IgG F(ab’)2 and anti-Swine Serum. No reaction was observed against anti-Swine IgG F(c) or anti-Pepsin. | |
Swine |
FITC | |
This product was prepared from normal serum by delipidation, salt fractionation and ion change chromatography followed by pepsin digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Swine IgG, anti-Swine IgG F(ab’)2 and anti-Swine Serum. No reaction was observed against anti-Swine IgG F(c) or anti-Pepsin. | |
Swine |
HRP | |
This product was prepared from normal serum by delipidation, salt fractionation, ion exchange chromatography followed by pepsin digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti- Swine IgG, anti-Swine IgG F(ab’)2 and anti-Swine Serum. No reaction was observed against anti-Swine IgG F(c) or anti-Pepsin. | |
Swine |
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