Streptavidin

• Catalog Number: orb348765
• Category: Tools
• Description: Streptavidin
• Tested applications: ChIP, ELISA, Gel Supershift Assays, SDS-PAGE, WB
• Concentration: 0.9 mg/mg
• Dilution range: ELISA: 1:20,000 - 1:200,000, ChIP: User Optimized, Gel Supershift Assays: User Optimized, WB: 1:2000-1:20,000
• Form/Appearance: Lyophilized
• Purity: Streptavidin is chromatographically pure and shows predominantly a single 53,600 dalton band by SDS-PAGE.
• Conjugation: Unconjugated
• Hazard Information: Non-Toxic
• UniProt ID: P22629
• Biological Activity: 15.5 U/mg by biotin titration method
• NCBI: CAA00084.1
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Streptavidin is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: Preservative: None. Stabilizer: None. 0.15 M Sodium Chloride
• Alternative names: SA, S avidin, streptococcus avidin
• Note: For research use only
• Application notes: Streptavidin has been tested by SDS-PAGE and is suitable for use as antigen, as a control or standard in assays, and most other immunological methods as well as enzyme conjugates and complexes; Southern blots and other methodologies related to DNA and RNA analysis; Western blots; and purification of proteins or other antigens with biotinylated antibodies or lectins by use of immobilized streptavidin.
• Expiration Date: 12 months from date of receipt.