Stra6 Antibody

SKU: orb1246668

Description

Stra6 Antibody

Research Area

Cell Biology

Images & Validation

• Tested Applications: ELISA, WB
• Reactivity: Mouse, Rat
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 80-90kDa band observed in Rat Testis lysates, which was successfully blocked by incubation with the immunizing peptide (calculated MW of 73.8kDa according to Rat NP_001025095.1). Recommended concentration: 1-2µg/mL. Primary incubation 1 hour at room temperature. An approx. 90kDa band was also observed in 3T3-L1 adipocytes reduced by stra6 siRNA. The 90kDa is stained after retrovirally overexpressing Mouse Stra6 and not after overexpressing Human Stra6 in 3T3 preadipocytes (calculated MW of 73.8kDa according to Mouse NP_033317.2). Primary incubation was 3 hours.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-QAFRKAALTSAKAN
• Target: Stra6
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

STRA6, stimulated by retinoic acid gene 6 homolog (mouse) , FLJ12541, MCOPS9, PP14296 , stimulated by retinoic acid gene 6 homolog

orb1246668 (1.7 ug/ml) staining of 3T3-L1 cell lysates (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1246668 (1 ug/ml) staining of Rat Testes lysate (A) + Peptide (B) (35 ug protein in RIPA buffer). Detected by chemiluminescence.

Quick Database Links

Gene Symbol

Stra6

UniProt

O70491

RefSeq (Protein):NP_001025095.1, NP_033317.2