STAT3 Antibody

SKU: orb345728

Description

STAT3 antibody

Research Area

Cancer Biology

Images & Validation

• Tested Applications: DOT, ELISA, WB
• Dilution Range: ELISA: 1:5000, WB: 1:500
• Reactivity: Human
• Application Notes:
  Anti-STAT3 antibody has been tested by Dot Blot and is useful for Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~88 kDa corresponding to STAT3 protein by Western Blotting in the appropriate cell lysate or extract.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: STAT3 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with synthetic monomethylated or asymmetric dimethylated peptides surrounding Arginine 31 of human STAT.
• Target: STAT3
• Purity: Anti-STAT3 was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody reacts with all STAT3 modifications. Cross-reactivity is seen against STAT1 and STAT4. BLAST analysis was used to suggest cross-reactivity with Human, mouse, and rat. Cross-reactivity with STAT3 from other sources has not been determined.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.05% (w/v) Sodium Azide. Stabilizer: 30% Glycerol; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/mL
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rabbit anti-STAT3 antibody, STAT-3, STAT 3, Signal transducer and activator of transcription 3, Acute-phase response factor, APRF

Dot blot of Rabbit Anti-STAT3 antibody. Antigen: non-modified, monomethylated and asymmetric dimethylated forms of the immunizing peptide from the internal portion of the STAT3 protein. Load: 100, 10, or 1 picomolar as indicated. Primary antibody: STAT3 (Internal) antibody at 1:1000 for 90 min at RT. (Exposure time: 3.6 seconds).

Quick Database Links

Gene Symbol

STAT3

UniProt

P40763