Stains-All

SKU: orb1307723

Description

Stains-All is a cationic carbocyanine dye used in biochemical research. It is commonly employed for the staining of proteins, nucleic acids, and proteoglycans in both in vitro assays and histological applications.

Research Area

Neuroscience

Key Properties

• CAS Number: 7423-31-6
• MW: 559.58
• Purity: 99.71% (May vary between batches)
• Formula: C₃₀H₂₇BrN₂S₂
• SMILES: [Br-].CCN1\C(Sc2ccc3ccccc3c12)=C\C(\C)=C\c1sc2ccc3ccccc3c2[n+]1CC
• Target: Others,CaMK
• Solubility: DMSO:9.5 mg/mL (16.98 mM)

Bioactivity

• In Vitro:
  Instructions 1. Preparation of stock solution and working solution: Stains-All can be dissolved in deionized water or organic solvents (such as ethanol); it is usually prepared at a working concentration of 0.5–5 µM, depending on the experimental conditions. 2. Staining steps: 1) Dissolve the target protein (such as calmodulin) in an appropriate buffer (such as PBS, pH 7.4). 2) Add an appropriate amount of Stains-All to the protein solution and mix gently. 3) Incubate at room temperature for 10–30 minutes to allow the dye to bind to the calcium binding site. 3. Fluorescence detection: Use a fluorescence spectrophotometer to excite at 630 nm and usually measure the emission spectrum at around 650 nm. 4. Data analysis: Compare the changes in fluorescence signals under different calcium concentrations to analyze the binding kinetics of calcium and calmodulin; in the titration experiment, the fluorescence response curve is drawn by gradually increasing the concentration of calcium ions, and then the binding constant is calculated. "

Storage & Handling

• Storage: keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

dye, carbocyanine, cationic, calcium-mimic, inhibit, Inhibitor, Stains All, StainsAll, Stains-All, stain

Compound Identifiers

PubChem CID

6364602