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Item 1 of 4
SREBF1 Antibody (Center)
Catalog Number: orb1928317
| Catalog Number | orb1928317 |
|---|---|
| Category | Antibodies |
| Description | Affinity Purified Rabbit Polyclonal Antibody (Pab) |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Clone Number | RB21265 |
| Tested applications | FC, IHC-P, WB |
| Predicted Reactivity | Hamster, Mouse, Rat |
| Reactivity | Human |
| Isotype | Rabbit IgG |
| Antibody Type | Primary Antibody |
| Dilution range | WB: 1:1000, WB: 1:1000, IHC-P: 1:10~50, FC: 1:10~50 |
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Conjugation | Unconjugated |
| MW | 121675 Da |
| Target | This SREBF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 349-378 amino acids of human SREBF1. |
| UniProt ID | P36956 |
| NCBI | NP_001005291.1, NP_004167.3 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Alternative names | Sterol regulatory element-binding protein 1, SREBP Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Western blot analysis of SREBF1 Antibody (Center) in MDA-MB435 cell line lysates (35 ug/lane). SREBF1 (arrow) was detected using the purified Pab.
SREBF1 Antibody (Center) flow cytometry analysis of Ramos cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Anti-SREBF1 Antibody (Center) at 1:1000 dilution + Jurkat whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 122 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Formalin-fixed and paraffin-embedded human breast carcinoma reacted with SREBF1 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
