SQSTM1/P62 Rabbit Polyclonal Antibody

SKU: orb1742580

Description

SQSTM1 antibody, also known as ubiquitin-binding protein p62 antibody, detects the SQSTM1 protein that interacts with LC3 proteins to execute autophagosomal degradation. SQSTM1 is also reported to regulate the activation of NFκB1 downstream signaling in addition to having other cellular functions.

Research Area

Cell Biology

Images & Validation

• Tested Applications: FC, ICC, IHC-P, IP, PLA, WB
• Dilution Range: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. FCM: 1:50-1:200. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
• Reactivity: Bovine, Human, Insect, Mouse, Rat, Zebrafish
• Application Notes:
  Positive control: NIH-3T3 , JC , BCL-1 , PC-12 , Rat2 , Huh7 ( 3μM Thapsigargin 12hr) , HepG2

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human SQSTM1 / P62. The exact sequence is proprietary.
• Target: sequestosome 1
• Molecular Weight: 48
• Purification: Purified by antigen-affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS, 1% rAlbumin, 20% Glycerol, 0.025% ProClin 300.
• Concentration: 0.39 mg/ml (Please refer to the vial label for the specific concentration.)
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

sequestosome 1 , A170 , DMRV , FTDALS3 , NADGP , OSIL , PDB3 , ZIP3 , p60 , p62 , p62B

Quick Database Links

Gene Symbol

sequestosome 1

UniProt

Q13501