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Item 1 of 4
SPHK2 Antibody (C-term)
Catalog Number: orb1929450
| Catalog Number | orb1929450 |
|---|---|
| Category | Antibodies |
| Description | Purified Rabbit Polyclonal Antibody (Pab) |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Clone Number | RB03625 |
| Tested applications | IHC-P, WB |
| Reactivity | Human |
| Isotype | Rabbit IgG |
| Antibody Type | Primary Antibody |
| Dilution range | WB: 1:1000, WB: 1:1000, IHC-P-Leica: 1:250, IHC-P-Leica: 1:250 |
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Conjugation | Unconjugated |
| MW | 69217 Da |
| Target | This SPHK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 590-620 amino acids from the C-terminal region of human SPHK2. |
| UniProt ID | Q9NRA0 |
| NCBI | NP_001191087.1, NP_001191089.1, NP_001191088.1, NP_064511.2 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Alternative names | Sphingosine kinase 2, SK 2, SPK 2, SPHK2 Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Immunohistochemical analysis on paraffin-embedded Human brain tissue was performed on the Leica BOND RXm. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:250) for 15 min at room temperature. Leica Bond Polymer Refine Detection was used as the secondary antibody.
Immunohistochemical analysis on paraffin-embedded Human kidney tissue was performed on the Leica BOND RXm. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:250) for 15 min at room temperature. Leica Bond Polymer Refine Detection was used as the secondary antibody.
SPHK2 Antibody (A605) western blot analysis in HepG2 cell line lysates (35 ug/lane). This demonstrates the SPHK2 antibody detected the SPHK2 protein (arrow).
All lanes: Anti-hSPHK2-A605 at 1:1000 dilution. Lane 1: HepG2 whole cell lysate. Lane 2: A549 whole cell lysate. Lane 3: 293 whole cell lysate. Lane 4: U-87 MG whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 69 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
