SPHK2 Antibody (C-term)

SKU: orb1929450

Description

Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Cancer Biology, Cell Biology, Immunology & Inflammation, Metabolism Research, Signal Transduction

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution Range: WB: 1:1000, WB: 1:1000, IHC-P-Leica: 1:250, IHC-P-Leica: 1:250
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: RB03625
• Target: This SPHK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 590-620 amino acids from the C-terminal region of human SPHK2.
• Molecular Weight: 69217 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Disclaimer: For research use only

Alternative Names

Sphingosine kinase 2, SK 2, SPK 2, SPHK2

Immunohistochemical analysis on paraffin-embedded Human brain tissue was performed on the Leica BOND RXm. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:250) for 15 min at room temperature. Leica Bond Polymer Refine Detection was used as the secondary antibody.

Immunohistochemical analysis on paraffin-embedded Human kidney tissue was performed on the Leica BOND RXm. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:250) for 15 min at room temperature. Leica Bond Polymer Refine Detection was used as the secondary antibody.

SPHK2 Antibody (A605) western blot analysis in HepG2 cell line lysates (35 ug/lane). This demonstrates the SPHK2 antibody detected the SPHK2 protein (arrow).

All lanes: Anti-hSPHK2-A605 at 1:1000 dilution. Lane 1: HepG2 whole cell lysate. Lane 2: A549 whole cell lysate. Lane 3: 293 whole cell lysate. Lane 4: U-87 MG whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 69 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Quick Database Links

Gene Symbol

This SPHK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 590-620 amino acids from the C-terminal region of human SPHK2.

UniProt

Q9NRA0

RefSeq (Protein):NP_001191088.1, NP_001191087.1, NP_064511.2, NP_001191089.1