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Item 1 of 6
Item 1 of 6
SNAP25 Recombinant Mouse Monoclonal Antibody
Catalog Number: orb1151970
| Catalog Number | orb1151970 |
|---|---|
| Category | Antibodies |
| Description | SNAP25 Recombinant Mouse Monoclonal Antibody |
| Species/Host | Mouse |
| Clonality | Recombinant |
| Tested applications | IF, IHC-Fr, IHC-P, WB |
| Predicted Reactivity | Mouse, Rat |
| Reactivity | Human, Mouse, Rat |
| Isotype | IgG1 |
| Immunogen | KLH conjugated synthetic peptide derived from human SNAP25 |
| Antibody Type | Recombinant Antibody |
| Concentration | 1mg/ml |
| Dilution range | WB=1:500-2000, IHC-P=1:20-100, IHC-F=1:400-800, IF=1:100-500 |
| Form/Appearance | Liquid |
| Conjugation | Unconjugated |
| MW | 23 kDa |
| Target | SNAP25 |
| UniProt ID | P60880 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Alternative names | SNAP-25; bA416N4.2; Bdr; dJ1068F16.2; FLJ23079; HG Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Blocking buffer: 5% NFDM/TBST, Primary ab Dilution: 1:2000, Primary ab incubation condition: 2 hours at room temperature, Lysate: Mouse brain, Rat brain, Protein loading quantity: 20 µg, Exposure time: 15 s, Predicted MW: 23 kDa, Observed MW: 25 kDa.
Paraformaldehyde-fixed, paraffin embedded (mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (SNAP25) Monoclonal Antibody, Unconjugated (orb1151970) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (SNAP25) Monoclonal Antibody, Unconjugated (orb1151970) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat cerebellum), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (SNAP25) Monoclonal Antibody, Unconjugated (orb1151970) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.
Tissue: Human cerebellum, Section type: Formalin fixed & Paraffin-embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary ab Dilution: 1:100, Primary ab incubation condition: 1 hour at room temperature, Counter stain: Hematoxylin, Comment: Color brown is the positive signal for orb1151970.
Tissue: Mouse cerebrall cortex, Section type: Formalin fixed & Paraffin-embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary ab Dilution: 1:100, Primary ab incubation condition: 1 hour at room temperature, Counter stain: Hematoxylin, Comment: Color brown is the positive signal for orb1151970.
