You have no items in your shopping cart.
Cart summary
Item 1 of 4
Item 1 of 4
SLC9A9 Recombinant Mouse Monoclonal Antibody
Catalog Number: orb1172830
| Catalog Number | orb1172830 |
|---|---|
| Category | Antibodies |
| Description | SLC9A9 Recombinant Mouse Monoclonal Antibody |
| Species/Host | Mouse |
| Clonality | Recombinant |
| Clone Number | H9B9 |
| Tested applications | ICC, IF, IHC-Fr, IHC-P, WB |
| Predicted Reactivity | Mouse, Rat |
| Reactivity | Human |
| Isotype | IgG2a/Kappa |
| Antibody Type | Recombinant Antibody |
| Concentration | 1mg/ml |
| Dilution range | WB=1:500-1:1000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:50, IF=0 |
| Form/Appearance | Liquid |
| Conjugation | Unconjugated |
| Target | SLC9A9 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Blocking buffer: 5% NFDM/TBST, Primary ab Dilution: 1:1000, Primary ab incubation condition: 4°C overnight, Secondary ab: Goat Anti-Mouse IgG H&L (HRP), Lysate: HepG2, PC-3, PC-12, EL4.IL-2, Protein loading quantity: 20 µg, Exposure time: 60 s, Predicted MW: 73 kDa, Observed MW: 73 kDa.
Cell line: HepG2, Fixative: 4% Paraformaldehyde, Permeabilization: 0.1% TritonX-100, Primary ab Dilution: 1:50, Primary incubation condition: 4°C overnight, Secondary ab: Goat Anti-Mouse IgG, Nuclear counter stain: DAPI (Blue), Comment: Color green is the positive signal for orb1172830.
Tissue: Human liver cancer, Section type: Formalin fixed & Paraffin-embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary ab Dilution: 1:100, Primary ab incubation condition: 1 hour at room temperature, Secondary ab: SP Kit (Mouse), Counter stain: Hematoxylin (Blue), Comment: Color brown is the positive signal for orb1172830.
Tissue: Human skeletal muscle, Section type: Formalin fixed & Paraffin-embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary ab Dilution: 1:100, Primary ab incubation condition: 1 hour at room temperature, Secondary ab: SP Kit (Mouse), Counter stain: Hematoxylin (Blue), Comment: Color brown is the positive signal for orb1172830.
