SKP1 Antibody

SKU: orb750504

Description

SKP1 antibody

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 1:2,000 - 1:10,000, WB: 1:500 - 1:1,000
• Reactivity: Human
• Application Notes:
  This antibody reacts with human SKP1 by western blot and immunoprecipitation. The antibody immunoprecipitates in vitro translated protein and protein from cell lysates (using HeLa, NIH-3T3, and others). Coimmunoprecipitation of cyclin A and Cuol1 may occur. An 18.5 kDa band corresponding to human SKP1 is detected. Most cell lines expressing SKP1 can be used as a positive control. Researchers should determine optimal titers for other applications.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Antiserum
• Immunogen: This antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 152-163 of Human SKP1 (C-terminus) coupled to KLH.
• Target: SKP1
• Purity: This product is monospecific antiserum processed by delipidation and defibrination followed by sterile filtration. This product reacts with human SKP1. Cross reactivity is expected against mouse SKP1 based on a high degree of sequence homology. Cross reactivity with other SKP1 proteins or SKP1 from other sources is not known.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Concentration: 85 mg/mL
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rabbit anti-SKP1 Antibody, Cyclin A/CDK2 associated protein p19 antibody, EMC 19 antibody, OCP 2 antibody, p19skp1 antibody, RNA polymerase II elongation factor like protein antibody, S phase kinase associated protein 1 antibody

Most modifiers mature by proteolytic processing from inactive precursors (a; amino acid). Arrowheads point to the cleavage sites. Ubiquitin is expressed either as polyubiquitin or as a fusion with ribosomal proteins. Conjugation requires activating (E1) and conjugating (E2) enzymes that form thiolesters (S) with the modifiers. Modification of cullins by RUB involves SCF (SKP1/cullin-1/F-box protein) / CBC (cullin-2/elongin B/elonginC) -like E3 enzymes that are also involved in ubiquitination. In contrast to ubiquitin, the UBLs do not seem to form multi-UBL chains. UCRP(ISG15) resembles two ubiquitin moieties linked head-to-tail. Whether HUB1 functions as a modifier is currently unclear. APG12 and URM1 are distinct from the other modifiers because they are unrelated in sequence to ubiquitin.

Western Blot of Anti-SKP1 Antibody. Cyclin F binds to Vif through the CY motif in the C-terminal region of HIV-1 NL4-3 Vif. (B) depiction of CY mutant generation of Vif (upper panel). Cyclin F does not co-immunoprecipitate CY mutant Vif (lower panel). Cyclin F-Skp1 interaction is found to be intact, but cyclin F-Vif interaction is lost when CY mutant of Vif is co-transfected with cyclin F in transfected 293T cells. All panels represent data from at least two or more independent experiments. IP, immunoprecipitate; IB, immunoblot.

Western Blot of Anti-SKP1 Antibody. Cyclin F physically interacts with Vif during HIV-1 infection in T cells. (B) cyclin F and Vif interacts endogenously during HIV-1 infection in 0.5 m.o.i. infected CEM-GFP (72 hpi) cells. Skp1 is also co-immunoprecipitated with cyclin F and Vif pulldown. All panels are representative of at least three independent experiments. IP, immunoprecipitation; IB, immunoblot.

Quick Database Links

Gene Symbol

SKP1

UniProt

P63208

RefSeq:52783797