SIRT3 Antibody (C-term)

• Catalog Number: orb1930162
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB01967
• Tested applications: IHC-P, WB
• Reactivity: Human, Mouse
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:2000, WB: 1:1000, WB: 1:1000, WB: 1:1000, IHC-P: 1:50~100
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 43573 Da
• Target: This SIRT3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 250-279 amino acids from the C-terminal region of human SIRT3.
• UniProt ID: Q9NTG7
• NCBI: NP_001017524.1, NP_036371.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: NAD-dependent protein deacetylase sirtuin-3, mitoc
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

SIRT3 Antibody (C-term) western blot analysis in KG-1 cell line lysates (35 ug/lane). This demonstrates the SIRT3 antibody detected the SIRT3 protein (arrow).

SIRT3 Antibody (C-term) western blot analysis in HepG2 cell line lysates (35 ug/lane). This demonstrates the SIRT3 antibody detected the SIRT3 protein (arrow).

Western blot analysis of lysates from 293, HepG2 cell line (from left to right), using SIRT3 Antibody (C-term). Diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35 ug per lane.

All lanes: Anti-SIRT3 Antibody (C-term) at 1:2000 dilution. Lane 1: human kidney lysate. Lane 2: human liver lysate. Lane 3: mouse liver lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 44 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.