Sinefungin

SKU: orb1217846

Description

Sinefungin is an effective inhibitor of virion mRNA(guanine-7-)-methyltransferase, mRNA(nucleoside-2'-)-methyltransferase, and viral multiplication. Sinefungin is a SET7/9 inhibitor and ameliorates renal fibrosis by inhibiting H3K4 methylation.

Key Properties

• CAS Number: 58944-73-3
• MW: 381.39
• Purity: >98% (HPLC)
• Formula: C₁₅H₂₃N₇O₅
• SMILES: N[C@H](CC[C@H](C(O)=O)N)C[C@@H]([C@@H]([C@@H]1O)O)O[C@@H]1n1c2ncnc(N)c2nc1
• Target: SET7/9
• Solubility: H2O:95 mg/mL (249.09 mM; Need ultrasonic)

Bioactivity

• In Vivo:
  Sinefungin (10 mg/kg, per day immediately after UUO) ameliorates renal fibrosis in obstructive nephropathy. Animal model: Male C57BL/6J mice (8 weeks of age). Dosage: 10 mg/kg. Administration: Administered intraperitoneally per day immediately after UUO (prepared as a suspension in distilled water and 0.9% NaCl solution). Result: Inhibited α-SMA protein expression. Ameliorated those (α-SMA, FSP-1, collagen 1, collagen 3) both at 3 and 7 days after UUO.
• In Vitro:
  Sinefungin (0.5 or 1.0 μg/mL, 60 minutes) ameliorates the TGF-β1-induced increase of α-SMA and inhibits the upregulation of histone H3K4 monomethylation in renal epithelial cells and renal fibroblast cells. Western blot analysis. Cell line: Renal epithelial cells. Concentration: 0.5 or 1.0 μg/mL. Incubation time: Pretreatment 60 minutes before TGF-β1 (10 ng/mL). Result: Significantly reduced TGF-β1-inducedα-SMA protein expression and inhibited H3K4me1 in a dose-dependent manner in both NRK-52E and NRK-49F cells.

Storage & Handling

• Storage: Storage temperature: -20°C. Stability: ≥ 2 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Adenosyl-Ornithine | A-9145 | Antibiotic 32232RP

Quick Database Links

Gene Symbol

SET7/9