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SIN3A Antibody
SKU: orb1259345
Featured
Description
Images & Validation
−Item 1 of 3
| Tested Applications | ChIP, IF, IP, WB |
|---|---|
| Reactivity | Human, Mouse |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human SIN3A (NP_056292.1). |
| Target | SIN3A |
| Molecular Weight | Observed: 165kDa |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Liquid |
| Concentration | batch dependent |
| Disclaimer | For research use only |
Alternative Names
−SIN3A, DKFZP434K2235, FLJ90319, KIAA0700,
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Western blot analysis of extracts of various cell lines, using SIN3A antibody (orb1259345) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Immunofluorescence analysis of U2OS cells using SIN3A antibody (orb1259345). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200 ug extracts of HeLa cells using 3 ug SIN3A antibody (orb1259345). Western blot was performed from the immunoprecipitate using SIN3A antibody (orb1259345) at a dilution of 1:500.
Quick Database Links
Gene Symbol
SIN3A
UniProt
UniProt Details
− No UniProt data available
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Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IF
Immunofluorescence
IP
Immunoprecipitation
ChIP
Chromatin Immunoprecipitation
SIN3A Antibody (orb1259345)
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