SIN3A Antibody

• Catalog Number: orb1259345
• Category: Antibodies
• Description: SIN3A Antibody
• Target: SIN3A
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Form/Appearance: Liquid
• Concentration: batch dependent
• Buffer/Preservatives: Liquid
• Purification: Affinity purification
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human SIN3A (NP_056292.1).
• UniProt ID: Q96ST3
• MW: Observed: 165kDa
• Tested applications: ChIP, IF, IP, WB
• Application notes: WB: 1:500 - 1:2000IF: 1:50 - 1:200IP: 1:50 - 1:100ChIP: 1:20 - 1:100
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: SIN3A, DKFZP434K2235, FLJ90319, KIAA0700,
• Research Area: Cancer Biology, Neuroscience
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using SIN3A antibody (orb1259345) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunofluorescence analysis of U2OS cells using SIN3A antibody (orb1259345). Blue: DAPI for nuclear staining.

Immunoprecipitation analysis of 200 ug extracts of HeLa cells using 3 ug SIN3A antibody (orb1259345). Western blot was performed from the immunoprecipitate using SIN3A antibody (orb1259345) at a dilution of 1:500.