Sheep IgM Antibody

SKU: orb346371

Description

Sheep IgM Antibody

Research Area

Protein Biochemistry

Images & Validation

• Tested Applications: SDS-PAGE
• Application Notes:
  Sheep IgM whole molecule has been tested in SDS-Page and can be utilized as a control or standard reagent in Western Blotting and ELISA experiments. Sheep IgM whole molecule is stable at 4° C prior to restoration. It is recommended to aliquot restored Sheep IgM whole molecule after adding equal parts glycerol for storage at -20° C for extended storage and to prevent repeated freeze-thaw cycles.

Key Properties

• Source: Sheep
• Biological Origin: Sheep
• Isotype: IgM
• Conjugation: Unconjugated
• Purity: Sheep IgM whole molecule was prepared from normal serum by a multi-step process which includes delipidation, selective precipitation and tandem molecular sieve chromatography followed by extensive dialysis against the buffer stated above. Sheep IgM whole molecule was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Sheep Serum and anti-Sheep IgM (µ chain specific). No reaction was observed against anti-Sheep IgG F(c). Some light chain cross reactivity will occur with anti-Sheep IgG.

Storage & Handling

• Storage: Store vial at 4° C prior to opening. This product is stable 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.1% (w/v) Sodium Azide; Buffer: 0.1 M Tris Chloride, 0.5 M Sodium Chloride, pH 8.0
• Concentration: 1.0 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Sheep Immunoglobulin M

SDS-Page of Sheep IgM Whole Molecule. Lane 1: Sheep IgM, Non-Reduced. Lane 2: Sheep IgM, Reduced. Load: 1.0 µg per lane. Predicted/Observed size-Predicted/Observed size - Non-Reduced: 900 kDa (Pentamer), 900 kDa (Molecule larger than can pass through gel), Reduced: 78 and 25 kDa, 75 and 25 kDa.