SGC707

SKU: orb1304558

Description

SGC707 is a cell-permeable, allosteric small molecule that potently and selectively inhibits PRMT3. It serves as a valuable chemical probe for investigating PRMT3's role in ribosome biogenesis and cellular differentiation in both in vitro and in vivo research contexts.

Research Area

Epigenetics & Chromatin

Key Properties

• CAS Number: 1687736-54-4
• MW: 298.34
• Purity: 99.89% (May vary between batches)
• Formula: C₁₆H₁₈N₄O₂
• SMILES: O=C(NCC(=O)N1CCCC1)Nc1ccc2cnccc2c1
• Target: Histone Methyltransferase
• Solubility: DMSO:125 mg/mL (418.99 mM);10% DMSO+40% PEG300+5% Tween 80+45% Saline:2.5 mg/mL (8.38 mM);Ethanol:55 mg/mL (184.35 mM);H2O:< 1 mg/mL (insoluble or slightly soluble)

Bioactivity

• Target IC50:
  PRMT3:31 nM|PRMT3:53 nM(Kd)
• In Vivo:
  In CD-1 male mice, SGC707 (30 mg/kg, i.p.) gives good plasma exposure over 6 h with the peak plasma level of 38000 nM, which suggests that SGC707 is suitable for animal studies.
• In Vitro:
  In cells, SGC707 binds to PRMT3 and reduces PRMT3-dependent H4R3me2a. SGC707 also stabilizes PRMT3 in both HEK293 and A549 cells with EC50 values of 1.3 μM and 1.6 μM, respectively.
• Cell Research:
  Cells are seeded in 96-well plates and treated with different concentrations of SGC707 for 72 h. Cell viability is determined using Alamar blue 0.01 mg/mL in the media. Resazurin reduction is monitored with 544 nm excitation, measuring fluorescence at 590 nm. (Only for Reference)

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

PRMT3, SGC707, SGC-707, SGC 707, inhibit, Histone Methyltransferase, HistoneMethyltransferase, Inhibitor

Compound Identifiers

PubChem CID

90642938