SETD8 Antibody (Center)

• Catalog Number: orb1929991
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB18964
• Tested applications: FC, IHC-P, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:1000, IHC-P: 1:50~100, FC: 1:10~50
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Conjugation: Unconjugated
• MW: 42890 Da
• Target: This SETD8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 220-249 amino acids from the Central region of human SETD8.
• UniProt ID: Q9NQR1
• NCBI: NP_065115.3
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: N-lysine methyltransferase SETD8, 211-, H4-K20-HMT
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

SETD8 Antibody (Center) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

SETD8 Antibody (Center) IHC analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SETD8 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

Western blot analysis of SETD8 antibody (Center) in 293 cell line lysates (35 ug/lane). SETD8 (arrow) was detected using the purified Pab.