SERPINE1/PAI-1 Antibody

• Catalog Number: orb1539005
• Category: Antibodies
• Description: SERPINE1/PAI-1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, IHC-P, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: E.coli derived recombinant Human PAI-1.
• Dilution range: ELISA (0.5 - 2 µg/ml), IHC, IHC-P (5 µg/ml), WB (0.1 - 0.2 µg/ml)
• Conjugation: Unconjugated
• Target: SERPINE1 / PAI-1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Lyophilized from PBS, pH 7.2. Lyophilized from PBS, pH 7.2
• Alternative names: SERPINE1, PAI1, PLANH1, PAI-1, Serpin E1
• Note: For research use only
• Application notes: Resuspension: Reconstitute with sterile water to 0.1-1.0 mg/ml.. Further information: Immunohistochemistry: LS-B7483 in an antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 5 ug/ml is recommended. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of hPAI-1 (4.5 ug/ml), a concentration of at least 15 ug/ml of this antibody is required. ELISA: To detect hPAI-1 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human PAI-1 (LS-C104564) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hPAI-1. Western Blot: To detect hPAI-1 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hPAI-1 is 1.5-3 ng/lane, under either reducing or non-reducing conditions.
• Expiration Date: 12 months from date of receipt.