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SERPINB1 Antibody

SKU: orb1535314

Description

SERPINB1 Antibody

Images & Validation

Tested ApplicationsELISA, IHC, IHC-P, WB
Dilution RangeIHC, IHC-P (5 µg/ml), Peptide-ELISA (1:64000), WB (0.5 - 2 µg/ml)
ReactivityHuman, Monkey
Application Notes
Further information: Peptide ELISA: antibody detection limit dilution 1:64000. Western blot: Approx 42-45kD bands observed in Human Placenta lysates (calculated MW of 42.7kD according to NP_109591.1). The fainter 45kD band likely represents the acetylated form. Both bands are blocked by the immunizing peptide. Recommended concentration: 0.5-2 ug/ml.

Key Properties

HostGoat
ClonalityPolyclonal
ImmunogenPeptide with sequence C-KTINQWVKGQTEGK, from the internal region of the protein sequence according to NP_109591.1.
TargetSERPINB1
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesTBS, pH 7.3, 0.02% Sodium Azide, 0.5% rAlbumin
Concentration0.5 mg/ml
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

SERPINB1, Anti-elastase, EI, LEI, Leukocyte elastase inhibitor, Peptidase inhibitor 2, PI-2, MNEI, ELANH2, M/NEI, PI2, Serpin B1

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Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

SERPINB1 Antibody

SERPINB1 antibody (0.5 ug/ml) staining of Human Placenta lysate (35 ug protein in RIPA buffer) with (B) and without (A) blocking with the immunizing peptide. Primary incubation was 1 hour. Detected by chemiluminescence.

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
IHC-P
Immunohistochemistry Paraffin
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

SERPINB1 Antibody (orb1535314)

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50 μg
$ 670.00
Free Secondary Antibody (20 ul)0/0

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