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Semaphorin 4A Recombinant Protein
Description
Images & Validation
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| Application Notes |
|---|
Key Properties
−| Source | Human Cells |
|---|---|
| Target | Sema4a |
| Tag | C-6 His tag |
| Molecular Weight | 72.7 kD |
| Protein Sequence | Thr33-His682 |
| Purity | Greater than 95% as determined by reducing SDS-PAGE.Endotoxin level less than 0.1 ng/ug (1 IEU/ug) as determined by LAL test. |
Storage & Handling
−| Storage | Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at -20°C for 3 months. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | Lyophilized from a 0.2 um filtered solution of 20mM PB,150mM NaCl,pH7.4. It is not recommended to reconstitute to a concentration less than 100 ug/ml. Dissolve the lyophilized protein in ddH2O. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Similar Products
−Recombinant Mouse SEMA4A Protein [orb2994529]
Unconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 72.7 KDa. Observed: 70-90 KDa, reducing conditions
10 μg, 50 μg, 500 μg, 1 mgSemaphorin 4A Recombinant Protein [orb1227878]
WB
>95% as determined by SDS-PAGE.
72.7 kDa
HEK293 cells
0.1 mgMouse SEMA4A protein [orb392435]
ELISA, MS, SDS-PAGE, WB
Greater than 95% as determined by reducing SDS-PAGE.
Human cells
50 μg, 500 μg, 10 μgSemaphorin-4A Recombinant Protein [orb1229873]
>98% (SDS-PAGE). Endotoxin level is less than 0.06EU/ μg protein (LAL test; Lonza).
CHO cells
100 μg

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Protocol Information
Semaphorin 4A Recombinant Protein (orb1228586)
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